diff --git a/tox.ini b/tox.ini
index 99448527..041c0e54 100644
--- a/tox.ini
+++ b/tox.ini
@@ -8,7 +8,6 @@ skip_missing_interpreters = True
 
 [testenv]
 setenv = VIRTUAL_ENV={envdir}
-         PYTHONHASHSEED=0
          CHARM_DIR={envdir}
          AMULET_SETUP_TIMEOUT=5400
 install_command =